ANR Analytical Lab 

525

525 TOTAL NITROGEN IN BOTANICAL MATERIALS - COMBUSTION METHOD (LECO FP528)

N
Total Nitrogen by Nitrogen Gas Analyzer utilizing induction furnace and thermal conductivity (LECO FP-528).

Summary: This method quantitatively determines the amount of nitrogen in all forms (ammonium, nitrate, protein and heterocyclic nitrogen) in botanical materials using an induction furnace and a thermal conductivity detector. Samples are ignited in an induction furnace at approximately 900°C, in helium and oxygen environment in a quartz combustion tube. An aliquot of the combustion gases is passed through a copper catalyst to remove oxygen and convert nitrous oxides to N2, scrubbed of moisture and carbon dioxide, and nitrogen content determined by thermal conductivity. The method has a detection limit of 0.02% nitrogen.

 
Sample amount requested: 3 g allows for full quality control.
Questions concerning limited sample size can be answered by the DANR Analytical Laboratory.

AOAC Official Method 990.03. Protein (Crude) in Animal Feed, Combustion Method, in Official Methods of Analysis of AOAC International, 18th Edition (2005), Chapter 4, pp. 30-31. AOAC International, Arlington, VA.